Hi all,
I want to identify conserved exons present in 3 eukaryotic genomes. I have the complete genomic scaffolds, predicted transcripts, and predicted proteins. My goal is to design degenerate primers that will amplify relatively conserved exons for a phylogenetic study.
Can anyone suggest a good strategy for doing this?
Best,
Kevin
I want to identify conserved exons present in 3 eukaryotic genomes. I have the complete genomic scaffolds, predicted transcripts, and predicted proteins. My goal is to design degenerate primers that will amplify relatively conserved exons for a phylogenetic study.
Can anyone suggest a good strategy for doing this?
Best,
Kevin
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