Hi all,
We replaced our Hiseq1000 with a NextSeq, largely due to the expense of the HS maintenance contract and the long run time. Our installation and training runs seemed fine, and I'm trying to assess how good our first 'real' run is. One thing I noticed is that reads are no longer of uniform length (44-152 nt on a 2x150 run). Another is that quality score binning is the default (which is okay for our purposes). I see quite a lot of N tails. We are planning to use it for 16s v6 sequencing, at a max of 1/6th of the flowcell combined with high diversity metagenomic or RNAseq libraries. Longer amplicons go on the Miseq, as before.
I'd be interested to hear early impressions from others who have made the switch and if there are any other tricks to moving Hiseq protocols to the Nextseq.
Thanks,
Hilary
We replaced our Hiseq1000 with a NextSeq, largely due to the expense of the HS maintenance contract and the long run time. Our installation and training runs seemed fine, and I'm trying to assess how good our first 'real' run is. One thing I noticed is that reads are no longer of uniform length (44-152 nt on a 2x150 run). Another is that quality score binning is the default (which is okay for our purposes). I see quite a lot of N tails. We are planning to use it for 16s v6 sequencing, at a max of 1/6th of the flowcell combined with high diversity metagenomic or RNAseq libraries. Longer amplicons go on the Miseq, as before.
I'd be interested to hear early impressions from others who have made the switch and if there are any other tricks to moving Hiseq protocols to the Nextseq.
Thanks,
Hilary
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