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04-18-2020, 08:31 AM
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#1 |
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Senior Member
Location: Oxford, Ohio Join Date: Mar 2012
Posts: 253
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MiSeq run failed; low clustering, but good QC
Have a strange situation, our latest MiSeq run failed for 16S - from a protocol (EMP @ ArgonneNatLabs) that we use all the time.
The clustering was unusually low (180 vs the expected 800) and we did a KAPA just prior to dilutions (those are correct, I checked). The run QC, Clusters %PF and all the obvious look very good - when the run finished, it did not demultiplex and says "failed". I've contacted Illumina, but this being a Saturday, I thought I'd see if anyone has seen this before? |
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04-23-2020, 09:32 AM
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#2 |
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Senior Member
Location: Oxford, Ohio Join Date: Mar 2012
Posts: 253
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Bad NaOH...
Made fresh & reloaded - now working. |
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| Tags |
| 16s, failed run, miseq 250bp |
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