16S & 18S Deep Sequencing Request(s)

cement_head · 08-04-2020, 09:40 AM

Hello,

We've done 16S/18S/ITS/amplicon sequencing for years, with good results - sometimes excellent (library dependent) - for both internal and external clients.

We're starting to see more and more requests for guaranteed extremely high read depth per sample - like in the neighbourhood of 50,000 to 100,000 reads per side per PE sample. What kind of Illumina instrument (other than a MiSeq) does this (amplicon) sequencing well and why are people becoming obsessed with ultra-high read depth?

TIA

KevinLam · 08-04-2020, 10:47 PM

Hmm e-dna?
environmental DNA?
deep metagenomics?

I think Ion Torrent does a fairly good job of this ..

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08-04-2020, 09:40 AM	#1
cement_head Senior Member Location: Oxford, Ohio Join Date: Mar 2012 Posts: 253	16S & 18S Deep Sequencing Request(s) Hello, We've done 16S/18S/ITS/amplicon sequencing for years, with good results - sometimes excellent (library dependent) - for both internal and external clients. We're starting to see more and more requests for guaranteed extremely high read depth per sample - like in the neighbourhood of 50,000 to 100,000 reads per side per PE sample. What kind of Illumina instrument (other than a MiSeq) does this (amplicon) sequencing well and why are people becoming obsessed with ultra-high read depth? TIA

08-04-2020, 10:47 PM	#2
KevinLam Senior Member Location: SEA Join Date: Nov 2009 Posts: 203	Hmm e-dna? environmental DNA? deep metagenomics? I think Ion Torrent does a fairly good job of this .. __________________ http://kevin-gattaca.blogspot.com/