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Old 10-30-2018, 04:56 PM   #1
cement_head
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Default Non-Standard bases from RTA

Weird question, does Illumina's current basecaller (RTA) on HiSeq3000 call non-standard bases? In other words, can you get something other than AGCT in the sequence read?
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Old 10-31-2018, 11:01 AM   #2
kmcarr
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Quote:
Originally Posted by cement_head View Post
Weird question, does Illumina's current basecaller (RTA) on HiSeq3000 call non-standard bases? In other words, can you get something other than AGCT in the sequence read?
Yes. An 'N'. Other than that no.
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