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Old 10-08-2018, 12:55 AM   #1
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Default Paired end dna seq for illumina

Why do we add A bases to 3'end post repair after shearing?
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Old 10-08-2018, 05:50 PM   #2
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To be able to add single-T-tailed adapters like the Illumina Truseq adapters via ligation. This is a way to prevent (or strongly reduce) insert-to-insert ligation and adapter-to-adapter ligation.
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