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Old 11-07-2018, 06:01 AM   #1
NGSMicro
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Default What volume of adapters do you get in the nextera XT kits?

Hello,

At our lab we have a custom protocol for adding indices to our amplicons, using only 5uL of each index when using the "Nextera XT Index Kit (24 indexes, 96 samples)".

I want to update this to use the "IDT® for Illumina® Nextera™ DNA UD Indexes Set A (96 Indexes, 96 Samples)" kit, but I can't find out how much (approximately) you get in each kit for each pair of indices.

Has anyone bought this kit?
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Old 11-08-2018, 02:47 AM   #2
nucacidhunter
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10 ul with some overage in each well which contains pool of two primers.
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Old 11-08-2018, 03:04 AM   #3
NGSMicro
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Thank you, most appreciated.
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