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I am preparing RNA libraries using the TruSeq Total RNA kit from Illumina and I am trying to use NextFlex adapters in lieu of the truseq ones. I am stuck on the correct concentration to use and Illumina tells me the molarity of their adapters is proprietary. Does anyone know the correct concentration of their adapters, I don't have any on hand to test.
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Check the concentration of the Nextflex RNA-seq adapters on their web page; I have not memorized it but it will work. -
Thanks Luc, I know the concentration of the nextflex adapters and I know they will work. I am looking for the concentration of the illumina adaptersComment
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Nextflex adapters are available for DNA applications and for RNA-seq applications. The latter are merely dilutions of the former, but Bioo provides the concentration info somewhere.
If you have the diluted Nextflex adapters (for RNA-seq) use the same adapter volume as in the TruSeq RNA kit.Comment
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