Can anybody here explain the molecular biology behind Ion Torrent RNA library prep? The protocol is particularly vague about adapter hybridization and ligation which is what I would like to understand.
According to the protocol, first the adapters are annealed and then ligated. Does this mean that the adapters are partly double stranded or a hairpin?
According to the protocol, first the adapters are annealed and then ligated. Does this mean that the adapters are partly double stranded or a hairpin?
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