Hello,
Anyone used Clontech Low Input Library Prep Kit (Cat. No. 634947)? It is suggested for the lib prep following cDNA synthesis/amplification with the SMARTer RNA-seq kit. But I think it can also be used for ChIP-seq or other lib prep which starts from small amount of DNA. The performance of this kit, according to the manual, is really impressive – it generates library that is enough for Illumina sequencing from 100 pg of dsDNA with only 14 cycles of PCR amplification, and all the reactions are done in a single tube. Is it possible? Does anyone has some clue how this kit works?
Best,
Li
Anyone used Clontech Low Input Library Prep Kit (Cat. No. 634947)? It is suggested for the lib prep following cDNA synthesis/amplification with the SMARTer RNA-seq kit. But I think it can also be used for ChIP-seq or other lib prep which starts from small amount of DNA. The performance of this kit, according to the manual, is really impressive – it generates library that is enough for Illumina sequencing from 100 pg of dsDNA with only 14 cycles of PCR amplification, and all the reactions are done in a single tube. Is it possible? Does anyone has some clue how this kit works?
Best,
Li
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