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Old 08-02-2017, 11:15 AM   #1
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Location: Tampa, Florida

Join Date: Aug 2011
Posts: 7
Default Why Ion Torrent use the Taqman qPCR for sequencing lib quantification

Hi All,

To be honest, I really do not understand why Ion torrent use the Taqman for library quantification (ION LIBRARY TAQMAN™ QUANTITATION KIT). I asked several people and a FAS, they had no idea. The point is how they design a probe for the assay? I tried to find a manual but failed. Can anybody explain it to me?. Thanks.

All the best.
chaomeizhang is offline   Reply With Quote
Old 08-03-2017, 03:42 AM   #2
Location: Norway

Join Date: Oct 2012
Posts: 13

IIRC the probe binds within the P1 adapter, that's why the kit is not compatible with the use of fusion primers with truncated P1.
torben is offline   Reply With Quote

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