Genome Sequencing Service that provides raw, untrimmed and unassembled output data?

newkid · 10-28-2012, 07:13 PM

Are there service providers that can just run purified, and amplified DNA through a sequencer and provide me with the output?

The data won't need to be trimmed, or assembled. Just looking for raw, single end, short bp reads.

Most service providers I've found seem to include assembly as a part of their service.

Any pointers or advice to help in my hunt would be much appreciated too.

GenoMax · 10-29-2012, 04:46 AM

A minimal amount of QC needs to happen as a part of the pre-processing of sequence data (generally via vendor software). You obviously do not want to get reads that are mixed (two clusters merged/close together) etc.. But other than that most providers should give you untrimmed full length sequence data files.

There is a list of service providers here: http://seqanswers.com/wiki/Service_Provider It does not appear to be working at the moment. Hopefully site admins will rectify that soon.

newkid · 10-29-2012, 07:41 PM

Looks like the link is up!

Thanks

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10-28-2012, 07:13 PM	#1
newkid Junior Member Location: California Join Date: Oct 2012 Posts: 9	Genome Sequencing Service that provides raw, untrimmed and unassembled output data? Are there service providers that can just run purified, and amplified DNA through a sequencer and provide me with the output? The data won't need to be trimmed, or assembled. Just looking for raw, single end, short bp reads. Most service providers I've found seem to include assembly as a part of their service. Any pointers or advice to help in my hunt would be much appreciated too.

10-29-2012, 04:46 AM	#2
GenoMax Senior Member Location: East Coast USA Join Date: Feb 2008 Posts: 7,088	A minimal amount of QC needs to happen as a part of the pre-processing of sequence data (generally via vendor software). You obviously do not want to get reads that are mixed (two clusters merged/close together) etc.. But other than that most providers should give you untrimmed full length sequence data files. There is a list of service providers here: http://seqanswers.com/wiki/Service_Provider It does not appear to be working at the moment. Hopefully site admins will rectify that soon.

10-29-2012, 07:41 PM	#3
newkid Junior Member Location: California Join Date: Oct 2012 Posts: 9	Looks like the link is up! Thanks