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Old 12-12-2007, 06:38 AM   #1
Michael L. Altshuler
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Location: Moscow

Join Date: Nov 2007
Posts: 13
Default Terms defining sequencing accuracy

Hi colleagues,

I am aware that with respect to accuracy the new generation methods can lag behind the Sanger’s.

The requirement of repeated sequencing of the same nucleotide stretch can apparently be expressed in different ways. What is the difference between “per base read accuracy” and “consensus accuracy”? Also between “sequencing coverage” and “bases covered %”. How these terms are connected with “sample depth”?

I have been unable to find satisfactory answers to these questions in Wikipedia. Is there any other free educational resource addressing this theme?

Thank you in advance,
Michael
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