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Old 10-26-2012, 01:03 PM   #1
rthornton4
Junior Member
 
Location: Houston, Texas

Join Date: Sep 2010
Posts: 6
Default Agilent SureSelect XT2 pre-capture library construction

Hi all,
I'm wondering if anyone has worked with the Agilent SureSelect XT2 protocol which features pre-capture library pooling. I am attempting this protocol for the first time and my individual pre-capture library yields were extremely low. I've previously worked with the Human All Exome protocol (no pre-capture library pooling) and have had no issues with my pre-capture library construction. I tried to amplify the pre-capture libraries here with the recommended 5 cycles of PCR and did not get the yields I expected. I was hoping someone else has had experience with this protocol and how many cycles of PCR were needed for their pre-capture libraries. Thanks in advance!!
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Old 01-30-2013, 05:17 AM   #2
scrosby
Director, GTAC, Washington U.
 
Location: St. Louis

Join Date: Nov 2009
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Default Pre-capture

We are looking at this pool as well, but have not yet made the decision to dive in (har-har). Has anyone had significant experience with pre-capture pooling?
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