Hi,
I am beginner in methylation studies. Recently I received HumanMethylation 450 BeadChip results that point to significant differences in methylation level between two human populations. Those methylation differences were placed beyond CpG island and covered only single CpG. Methylation difference wasn’t found in neighbouring CpG. It is quite strange for me because I expected that such population differences should be observed in bigger genome area- not in a single CpG. When I checked the sequence in Ensamble I have noticed that in this single CpG- nucleotide G is polymorphic in one of the population. Is anyone notice such situation or maybe have read about problem like this? Is it still a methylation studies or just polymorphism?
Many thanks!
Pat
I am beginner in methylation studies. Recently I received HumanMethylation 450 BeadChip results that point to significant differences in methylation level between two human populations. Those methylation differences were placed beyond CpG island and covered only single CpG. Methylation difference wasn’t found in neighbouring CpG. It is quite strange for me because I expected that such population differences should be observed in bigger genome area- not in a single CpG. When I checked the sequence in Ensamble I have noticed that in this single CpG- nucleotide G is polymorphic in one of the population. Is anyone notice such situation or maybe have read about problem like this? Is it still a methylation studies or just polymorphism?
Many thanks!
Pat
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