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RNA-Seq: Orientation and expression of methicillin-resistant Staphylococcus aureus sm Newsbot! Literature Watch 0 01-05-2011 02:00 AM

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Old 02-01-2012, 06:42 AM   #1
rdeborja
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Default Staph Aureus and Rhodobacter

Can anyone tell me if staph aureus and rhodobacter are diploid or haploid?
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Old 02-02-2012, 02:21 AM   #2
krobison
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I believe all bacteria are haploid in terms of allele content.

However, in rich medium, some bacteria are replicating very rapidly, and so the chromosome will appear to be at multiple copies near the origin of replication. I think this can be seen in sequencing depth plots, but can't remember where I saw it (unless it was in original Church sequencing-by-ligation paper)
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Old 02-02-2012, 01:25 PM   #3
swbarnes2
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Yeah, I don't see why bacteria wouldn't be haploid.

But that doesn't mean that every sample given to you is clonal.

Last edited by swbarnes2; 02-02-2012 at 01:47 PM. Reason: very stupid typo
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Old 02-12-2012, 09:06 PM   #4
ScottC
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Yes, theoretically, one chromosome copy per cell for (most) bacteria. But, as pointed out above, it doesn't always work that way.
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Old 02-20-2012, 04:14 AM   #5
kant1_IC
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If you sequence with a large cover and a huge deepth, you'll be able to see different clones.
That does not mean that your bacteria is polyploid.
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