Hello All!!
I am working on Histone acetylation in mammalian cells.... started with standardisation of Chip assay...
got stuck up with chromatin sonication.... when i run sonicated and unsonicated DNA on gel, i get smear for unsonicated control... and not the expected genomic DNA band that runs higher near the well. Can anybody xplain me why am i getting a smear instead of band of genomic DNA??
I am working on Histone acetylation in mammalian cells.... started with standardisation of Chip assay...
got stuck up with chromatin sonication.... when i run sonicated and unsonicated DNA on gel, i get smear for unsonicated control... and not the expected genomic DNA band that runs higher near the well. Can anybody xplain me why am i getting a smear instead of band of genomic DNA??