Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Illumina TruSeq Small RNA library preparation

    Hi, I'm some problems with micro RNA library preparation using Illumina TruSeq Small RNA protocol.
    I attach the images of bioanalyzer before gel purification of Ambion Human Brain Total RNA that I use as positive control.
    One images is about the control after 11 cycles of PCR Amplification (that is the cycles number that I usually use), but as you can see there is an evident decrease of material greater than approximately 100bp. We observe this kind of profile in all our samples and, in our experience, these are not normal patterns.
    The other image is the same control with 15 cycles of PCR amplification and is quite different.

    Are these patterns normal or there is any trouble?

    Have you any suggestion to solve the problems?

    Thanks in advance
    Attached Files

  • #2
    At 15 cycles, 147bp and 198bp bands reduced and 137bp and 184bp bands emerged. How did the gel purification go? On the gel also did you see similar pattern? Were you able to cutout the band excluding adapter dimer. However, i dont see adpter dimer band in this case. Thanks.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Strategies for Sequencing Challenging Samples
      by seqadmin


      Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
      03-22-2024, 06:39 AM
    • seqadmin
      Techniques and Challenges in Conservation Genomics
      by seqadmin



      The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

      Avian Conservation
      Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
      03-08-2024, 10:41 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 06:37 PM
    0 responses
    8 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, Yesterday, 06:07 PM
    0 responses
    8 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-22-2024, 10:03 AM
    0 responses
    49 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 03-21-2024, 07:32 AM
    0 responses
    67 views
    0 likes
    Last Post seqadmin  
    Working...
    X