Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation

Similar Threads
Thread Thread Starter Forum Replies Last Post
Changing HiSeq Parameters Mid Run coleen_2 Illumina/Solexa 4 12-11-2018 09:07 AM
Genome Res De novo bacterial genome sequencing: millions of very short reads assembly b_seite Literature Watch 1 10-05-2017 12:26 AM
A new fruitfly genome de novo sequencing nextomics De novo discovery 3 04-25-2012 07:53 AM
Getting started with de novo genome sequencing michaelbarton De novo discovery 2 01-23-2010 04:04 PM
illumina de novo txome assm: overlapping paired ends or long inserts? Zigster De novo discovery 1 10-15-2009 05:46 AM

Thread Tools
Old 02-26-2013, 06:39 AM   #1
Location: Japan

Join Date: Feb 2011
Posts: 12
Arrow mid-range inserts for HiSeq de novo genome sequencing

Hello. I wonder if any of you is familiar with the protocol to generate paired-end DNA library for illumina HiSeq in de novo genome sequencing.

I did paired-end sequencing with 200bp inserts, and want to try with longer inserts (e.g., 800bp, 2kb) to get higher continuity of assembly.

At PAG meeting in San Diego early this year, I heard about a series of libraries with different insert lengths used in the Parrot genome project, but have not found any publication and technical information for that project.
MesutOezil is offline   Reply With Quote
Old 02-26-2013, 07:31 AM   #2
Senior Member
Location: London

Join Date: Jun 2009
Posts: 298

I would also check out this post, where long inserts have been shown to still sequencing. This indicates that 800bp inserts should be fine (certainly on MiSeq).
TonyBrooks is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 07:03 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO