mid-range inserts for HiSeq de novo genome sequencing

MesutOezil · 02-26-2013, 06:39 AM

Hello. I wonder if any of you is familiar with the protocol to generate paired-end DNA library for illumina HiSeq in de novo genome sequencing.

I did paired-end sequencing with 200bp inserts, and want to try with longer inserts (e.g., 800bp, 2kb) to get higher continuity of assembly.

At PAG meeting in San Diego early this year, I heard about a series of libraries with different insert lengths used in the Parrot genome project, but have not found any publication and technical information for that project.

TonyBrooks · 02-26-2013, 07:31 AM

http://core-genomics.blogspot.co.uk/...-possible.html

I would also check out this post, where long inserts have been shown to still sequencing. This indicates that 800bp inserts should be fine (certainly on MiSeq).
http://seqanswers.com/forums/showthread.php?t=20839

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02-26-2013, 06:39 AM	#1
MesutOezil Member Location: Japan Join Date: Feb 2011 Posts: 12	mid-range inserts for HiSeq de novo genome sequencing Hello. I wonder if any of you is familiar with the protocol to generate paired-end DNA library for illumina HiSeq in de novo genome sequencing. I did paired-end sequencing with 200bp inserts, and want to try with longer inserts (e.g., 800bp, 2kb) to get higher continuity of assembly. At PAG meeting in San Diego early this year, I heard about a series of libraries with different insert lengths used in the Parrot genome project, but have not found any publication and technical information for that project.

02-26-2013, 07:31 AM	#2
TonyBrooks Senior Member Location: London Join Date: Jun 2009 Posts: 298	http://core-genomics.blogspot.co.uk/...-possible.html I would also check out this post, where long inserts have been shown to still sequencing. This indicates that 800bp inserts should be fine (certainly on MiSeq). http://seqanswers.com/forums/showthread.php?t=20839