Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • rRNA contamination and streptavidin beads

    Hello everyone!

    I recently prepared a RNA library (RNA extracted from cultured fibroblasts) and ran it on a NextSeq 500, using the Illumina TruSeq Stranded Total RNA Kit and using the Illumina Ribo-Zero rRNA Depletion Kit (legacy version, i.e. streptavidin pull-down) for rRNA depletion.

    In the results, I noticed that ~90% of all sequencing reads aligned to the ribosomal contig and therefore I suspected rRNA contamination. I then found out that the rRNA removal beads (Illumina) had been stored at -20°C for roughly 2 years. According to Illumina, storing these beads not at -20°C (instead of the recommended +4°C) could be a reason for rRNA contamination.

    My question is: could the freezing impair the binding capacity of the beads or has anybody made similar experiences?

    Thank you very much in advance for any help and best regards!

Latest Articles

Collapse

  • seqadmin
    Techniques and Challenges in Conservation Genomics
    by seqadmin



    The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

    Avian Conservation
    Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
    03-08-2024, 10:41 AM
  • seqadmin
    The Impact of AI in Genomic Medicine
    by seqadmin



    Artificial intelligence (AI) has evolved from a futuristic vision to a mainstream technology, highlighted by the introduction of tools like OpenAI's ChatGPT and Google's Gemini. In recent years, AI has become increasingly integrated into the field of genomics. This integration has enabled new scientific discoveries while simultaneously raising important ethical questions1. Interviews with two researchers at the center of this intersection provide insightful perspectives into...
    02-26-2024, 02:07 PM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 03-14-2024, 06:13 AM
0 responses
32 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-08-2024, 08:03 AM
0 responses
71 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-07-2024, 08:13 AM
0 responses
80 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-06-2024, 09:51 AM
0 responses
68 views
0 likes
Last Post seqadmin  
Working...
X