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  • #1

    Per Base Sequence Content

    Hi!
    I have 46 mRNA seq. in everyone the graph looks like this (Illumina):

    Click image for larger version Name: Screen Shot 2013-08-24 at 2.45.59 PM.png Views: 1 Size: 117.5 KB ID: 308222

    Can somebody explain why position 1-15 are not 25% of each?
    Tags: None
  • #2
    It has something to do with the random priming process not being quite so random in reality.

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    • #3
      I see this have been covered before:

      Trimming left end (5') of reads?? - SEQanswers
      http://seqanswers.com/forums/showthread.php?t=11843
      Application of sequencing to RNA analysis (RNA-Seq, whole transcriptome, SAGE, expression analysis, novel organism mining, splice variants)
      Last edited by sindrle; 08-24-2013, 08:22 AM.

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