MiSeq 16S reads (dumb question)

[Accentor]

Hello,

I've got a question that I assume is simple, despite my failed attempts to find the answer online...

I've received some 16s sequences from JGI (MiSeq), and I notice that some sequences in the .fastq file contain a "\1" immediately after the barcode. I assume this means that this sequence only appears once in the dataset, but I cannot find the answer anywhere.

Am I correct in assuming that these sequences are singletons?

Thanks.

[GenoMax]

If you can post an example header then we can be completely certain but in general "/1" generally indicates that the sequence belongs to Read 1 (from a pair in an paired end experiment). See illumina sequence header information in the FASTQ format entry on Wikipedia: http://en.wikipedia.org/wiki/FASTQ_format

[Accentor]

Yes, that is exactly what they are. Next time I need to go straight to Wikipedia

Thanks