Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • PubMed: Highly sensitive pyrosequencing system with polymer-supported enzymes for hig

    Syndicated from PubMed RSS Feeds

    Highly sensitive pyrosequencing system with polymer-supported enzymes for high-throughput DNA analysis.

    Anal Chem. 2011 Aug 21;

    Authors: Shiari M, Goto M, Suzuki S, Kono K, Kajiyama T, Kambara H

    Abstract
    A highly sensitive massively parallel pyrosequencing system employing a gel matrix to immobilize enzymes at high density in micro-reaction chambers is demonstrated. Reducing the size of micro-reaction chambers in a DNA analyzer is important to achieve a high-throughput utilizing a commercially available detection device or camera. A high-performance system can be attained by detecting signals from one reaction chamber with one photo-pixel of around several micrometers by utilizing 1:1 image magnification. However, the use of small beads immobilizing DNA has a disadvantage in detecting luminescence because only small amounts of DNA can be immobilized on the bead surfaces for sequencing. As luminescence intensity could be enhanced by increasing luciferase density in chambers, we overcame this difficulty by using a gel matrix to immobilize luciferase at a high concentration in micro-reaction chambers. One order of magnitude higher luminescence could be observed with the new method compared to the conventional method. Consequently, the chamber size and bead size immobilizing DNA could be reduced to as small as 6.5 ?m and 4-?m, respectively. This can be successfully applied to achieving small, inexpensive pyrosequencing systems with high throughput.


    PMID: 21854050 [PubMed - as supplied by publisher]



    More...

Latest Articles

Collapse

  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM
  • seqadmin
    Techniques and Challenges in Conservation Genomics
    by seqadmin



    The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

    Avian Conservation
    Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
    03-08-2024, 10:41 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 03-27-2024, 06:37 PM
0 responses
13 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-27-2024, 06:07 PM
0 responses
11 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-22-2024, 10:03 AM
0 responses
53 views
0 likes
Last Post seqadmin  
Started by seqadmin, 03-21-2024, 07:32 AM
0 responses
69 views
0 likes
Last Post seqadmin  
Working...
X