Hello everyone!
According to TruSight Rapid Capture lib prep method, 50ng (5ng/ul) input DNA is required in a final volume of 10ul for one library. So if we want to pool different libraries (Lets say 10) what should be the final concentration and volume of our pooled libraries?
Also, what should be the final concentration of DNA for optimum clustering?
Thanks!
According to TruSight Rapid Capture lib prep method, 50ng (5ng/ul) input DNA is required in a final volume of 10ul for one library. So if we want to pool different libraries (Lets say 10) what should be the final concentration and volume of our pooled libraries?
Also, what should be the final concentration of DNA for optimum clustering?
Thanks!
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