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Old 07-25-2013, 01:49 PM   #1
aparnanatarajan
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Location: St.louis

Join Date: Nov 2011
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Default miRNA library size selection using Ampure beads

Hi

I am trying to use AMPure XP beads to size select miRNA libraries. I am following the NEB protocol and the recommended cut off is 1.3x and 3.7x for smaller and larger respectively. I was wondering if anyone has tried this before and if so how stringent is the selection? The figure from NEB protocol shows a peak only at 147 removing all other fragments. Does this mean that the protocol needs to be tweaked every time we expect small RNAs other than miRNA?

Thank you.
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