I was uploading my RNA-seq assembly in TSA and I am not sure how to proceed. 743 sequences from my assembly got flagged with Code(VECTOR_MATCH) UniVec vector: gnl|uv|NGB00150.1:1-46 Ambion FirstChoice RLM-RACE 3' RACE adapter. I find it weird because I thought I already removed adapters prior to assembly using trimmomatic + fastqc. I have searched for the sequence of the adapter. What I want to know is how do I safely trim out the adapter sequence. I mean what if the vector is at the middle portion of the sequence, is it still acceptable to just remove the chunk and merge the two segments together? I could not find any sources to answer this at the moment that's why I am here.
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.
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Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...-
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