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I was uploading my RNA-seq assembly in TSA and I am not sure how to proceed. 743 sequences from my assembly got flagged with Code(VECTOR_MATCH) UniVec vector: gnl|uv|NGB00150.1:1-46 Ambion FirstChoice RLM-RACE 3' RACE adapter. I find it weird because I thought I already removed adapters prior to assembly using trimmomatic + fastqc. I have searched for the sequence of the adapter. What I want to know is how do I safely trim out the adapter sequence. I mean what if the vector is at the middle portion of the sequence, is it still acceptable to just remove the chunk and merge the two segments together? I could not find any sources to answer this at the moment that's why I am here.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Today, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM