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Illumina Primers
Hi everyone,
I am new to the SEQanswers community and have been searching through
the threads to find primer and adapter concentrations for use with the Illumina GA II system.
I am planning to do MeDIP-Seq for Paired-end multiplexed (index/tagged) samples.
In some of the other threads it is implied that the sequencing primers are 100um. However does this concentration apply to the adapters or the primers for LM-PCR ??
Also another thing has me slightly confused.
Some groups achieve multiplexing by designing tagged adapters, whereas others add the tag during LM-PCR. Is this because it's not too important when the tag goes in or are there specific advantages to either strategy.
Considered thoughts would be greatly appreciated.
Cheers
Will
I am new to the SEQanswers community and have been searching through
the threads to find primer and adapter concentrations for use with the Illumina GA II system.
I am planning to do MeDIP-Seq for Paired-end multiplexed (index/tagged) samples.
In some of the other threads it is implied that the sequencing primers are 100um. However does this concentration apply to the adapters or the primers for LM-PCR ??
Also another thing has me slightly confused.
Some groups achieve multiplexing by designing tagged adapters, whereas others add the tag during LM-PCR. Is this because it's not too important when the tag goes in or are there specific advantages to either strategy.
Considered thoughts would be greatly appreciated.
Cheers
Will
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