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Anyone preparing ITS amplicon libraries for use on a MiSeq platform? Care to share any of your primer designs? Need to start examining fungal communities and trying not to reinvent the wheel.
Thanks!
Thanks!
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I'm wondering the same thing. What did you end up doing? Any suggestions? -
Hi There,
I have the protocol for ITS Fungal in HiSeq working. New forward primer, better coverage than the previous described, we are preparing the paper now.
Cheers
EricComment
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I am currently designing Illumina fusion primers but am getting a bit worried. I don't want to get stuck buying 96 unique really long fusion primers (I want dual indexing). Do you know of anyone using complete fusion primers (adaptor+seq primer+index+PCR primer) VERSUS ligating adaptor+seq primer+index to standard PCR product after amplification?Comment
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Hi Eric, would like to check if your paper already published. If so, could you please send me the link and share the protocol &primers? Thank you so much!!Comment
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