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Old 10-07-2012, 01:55 PM   #21
JackieBadger
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Reads....there are many more clusters... Without the upgrade MiSeq produces up to 5 million reads.
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Old 10-08-2012, 09:25 AM   #22
kmcarr
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Quote:
Originally Posted by JackieBadger View Post
Reads....there are many more clusters... Without the upgrade MiSeq produces up to 5 million reads.
No, with the upgrade the output is 15-17 million CLUSTERS. This makes 30-34 million PE reads.
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Old 05-05-2016, 07:46 AM   #23
trauba
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What exactly is it that limits the read length anyways?
I understand imperfect cleavage of fluorescence reduces the # of extending oligos, lowering the signal + adding noise.
Also, eventually some oligos get "out of phase" and then muddy the signal. Is this somewhat caused by insufficient purging of reagents from previous cycles?

Thanks.
Jim
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