Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa

Similar Threads
Thread Thread Starter Forum Replies Last Post
45S rRNA copies ScampiZinc Bioinformatics 3 02-06-2015 08:02 AM
Diploid genomic copies actually sequenced charlescoldroom Sample Prep / Library Generation 0 01-10-2015 09:38 AM
Quantifying copies of a single locus robinweide Bioinformatics 0 10-17-2014 02:28 PM
Need help understanding Maximum Parsimony and Maximum Likelihood Giftedzipper Bioinformatics 0 01-09-2014 08:08 PM
Maximum cluster density for a GA and Hiseq flowcell that still produces good result Dr. Hybe Illumina/Solexa 3 03-31-2011 05:37 AM

Thread Tools
Old 05-04-2016, 03:52 PM   #1
Junior Member
Location: Salt Lake City, UT

Join Date: May 2016
Posts: 3
Default Maximum # of copies in a cluster?

Hi all,

I am just wondering why the # of DNA copies in each cluster is capped between 1000-2000.

Is this just due to the size of the well? I'm guessing that all fixed oligos in a well are extended during bridge amplification.

Or does the sequencing process fail w/ too many copies?


trauba is offline   Reply With Quote
Old 05-04-2016, 04:57 PM   #2
Senior Member
Location: US

Join Date: Dec 2010
Posts: 342

Only Illumina knows. I am guessing the isothermal clustering for the X10 and the HS4K is mostly limited by the real estate (and resulting steric problems for the bridged molecules?) and the number of flowcell oligos per nanowell.
luc is offline   Reply With Quote

amplification, cluster, copy number, nano, template

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 02:24 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO