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Old 11-21-2016, 02:36 PM   #1
Location: Alabama

Join Date: Jun 2009
Posts: 48
Default HiSeq 2500 rapid run versus high output

Hi all,

I'm taking advantage of an end-of-the-year RNA-Seq promotion from my sequencing provider. I was given the option of using their U.S. or overseas sequencing center. They said "if we were to do it here, we would be sequencing your samples on the HiSeq2500 in Rapid Mode rather than High Output mode." Apparently the turn-around time and data yield for each sample would be the same for either instrument. Any suggestions on which I should go after?

kmkocot is offline   Reply With Quote
Old 11-21-2016, 05:51 PM   #2
Jafar Jabbari
Location: Melbourne

Join Date: Jan 2013
Posts: 1,187

Average output of Rapid run is 150M/lane while HO is 250M and Gb of data from HO is cheaper than Rapid. Maybe they are selling reads not the lane. Data quality are similar so either should be fine.
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