Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • rate my tophat2 results :-)

    Hi,
    New in RNA-seq and without any help in my lab, i would like to ask you your advice about my mapping results. (In my opinion, it's ok, but i will be very happy if experimented poeple can confirm it or not).
    here are align summary tophat results and accepted_hits.bam analysed by samtool flagstat.

    Left reads:
    Input : 16641634
    Mapped : 15985940 (96.1% of input)
    of these: 4863655 (30.4%) have multiple alignments (7966 have >20)
    Right reads:
    Input : 16641634
    Mapped : 15922959 (95.7% of input)
    of these: 4834402 (30.4%) have multiple alignments (7972 have >20)
    95.9% overall read mapping rate.

    Aligned pairs: 15508899
    of these: 4652618 (30.0%) have multiple alignments
    87521 ( 0.6%) are discordant alignments
    92.7% concordant pair alignment rate.

    =============

    47082928 + 0 in total (QC-passed reads + QC-failed reads)
    0 + 0 duplicates
    47082928 + 0 mapped (100.00%:-nan%)
    47082928 + 0 paired in sequencing
    23593110 + 0 read1
    23489818 + 0 read2
    44621168 + 0 properly paired (94.77%:-nan%)
    45651258 + 0 with itself and mate mapped
    1431670 + 0 singletons (3.04%:-nan%)
    2790766 + 0 with mate mapped to a different chr
    86220 + 0 with mate mapped to a different chr (mapQ>=5)

    Thanks for your advice.

Latest Articles

Collapse

  • seqadmin
    Current Approaches to Protein Sequencing
    by seqadmin


    Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
    04-04-2024, 04:25 PM
  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 04-11-2024, 12:08 PM
0 responses
23 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 10:19 PM
0 responses
24 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-10-2024, 09:21 AM
0 responses
21 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-04-2024, 09:00 AM
0 responses
52 views
0 likes
Last Post seqadmin  
Working...
X