Hey people!
A colleague of mine wants to sequence a library of ssDNA Adeno-associated viruses on the MinION to get a distribution of their genome sizes and orientations.
To get the size distributions without fragmenting, we wanted to use the ligation kit from ONT which requires dsDNA. He wants to avoid a PCR step though, as to not lose the information about the genome orientations, as it would be essentially random which strand/orientation gets sequenced, right?
Is there a way to directly sequence ssDNA on the MinION? I found nothing so far, so possibly not.
A colleague of mine wants to sequence a library of ssDNA Adeno-associated viruses on the MinION to get a distribution of their genome sizes and orientations.
To get the size distributions without fragmenting, we wanted to use the ligation kit from ONT which requires dsDNA. He wants to avoid a PCR step though, as to not lose the information about the genome orientations, as it would be essentially random which strand/orientation gets sequenced, right?
Is there a way to directly sequence ssDNA on the MinION? I found nothing so far, so possibly not.
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