Go Back   SEQanswers > Sequencing Technologies/Companies > Ion Torrent

Similar Threads
Thread Thread Starter Forum Replies Last Post
Trusight Cancer panel olivervugrek Illumina/Solexa 0 02-05-2016 08:13 AM
cancer panel on miseq bartekd Illumina/Solexa 3 01-29-2013 01:56 PM
AmpliSeq Cancer Panel SarahNGS Ion Torrent 10 10-01-2012 10:21 AM

Thread Tools
Old 02-16-2017, 05:28 PM   #1
Junior Member
Location: Japan

Join Date: Jun 2015
Posts: 8
Default Colon and Lung Cancer Research Panel vs. Cancer Hotspot Panel


I plan to detect mutations from DNA derived from lung cancer FFPE sample with Ion PGM Ampliseq. I usually use Cancer Hotspot Panel v2. It works well, but sometimes I don't get a good result or not enough amount of reads to be analyzed.

I heard about this Colon and Lung Cancer Panel v2, and am thinking about using it. I know the difference is this one has less target gene (22 genes) compare to Cancer Hotspot Panel (50 genes). But besides that, is there any other difference? Can I use the same protocol? The price difference is quite big.

Please help, I'd really appreciate it.


Last edited by hanco; 02-16-2017 at 05:37 PM.
hanco is offline   Reply With Quote
Old 02-16-2017, 11:53 PM   #2
tristan dubos
Location: France

Join Date: Dec 2015
Posts: 39

I worked on the Colon and Lung Cancer Panel v2 for 1 years , i cant tell you that if DNA is good we can sequence around 300 000 reads per sample (we multiplex 16 samples per run). In still good condition we have more than 500X depth coverage per target which is our limit for somatic result. I will ask in my lab if the used Cancer Hotspot Panel before.

Hope it help a bit.

Best regards

tristan dubos is offline   Reply With Quote

ampliseq, cancer panel, cancer panel v2, ion pgm, ion torrent

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 11:44 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO