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06-21-2011, 03:03 PM
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#1 |
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Junior Member
Location: california Join Date: May 2011
Posts: 1
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Hi, one question about RNA-Seq library prep
Hi all,
I'm new in this field and want to learn something here.  I'm doing RNA-Seq library prep, we need to get good libraries from small starting total RNA material. When i tried to use 500ng total RNA to start, i usually couldn't get more than 1ng of purified cDNA. Does anyone have some suggestions on how to get more cDNA? Thanks -Flora |
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