Has anyone tried any of the automated size fractionation systems that are out there? They look like they could simplify and standardise a process that users spend a lot of time and effort on and as the number of libraies goes up gels are certainly not flavour of the month.
We have thought about bead based cleanup and size selection but the range is quite broad which does not fit all applications as well (miRNA, Structural Variation).
Pippin Prep: Sage Science
Lab ChIPXT: Caliper Life Science
We have thought about bead based cleanup and size selection but the range is quite broad which does not fit all applications as well (miRNA, Structural Variation).
Pippin Prep: Sage Science
- 4 samples on a ‘chip’
- 60-90 minute run time
- 40ul loading volume and up to 20ug of material,
- not sure on how tightly you can specify the size to collect.
- ‘Bioanalyser’-esque electropherogram
Lab ChIPXT: Caliper Life Science
- 4 samples on a ‘chip’
- 30 minute run time
- Not sure on loading volumes
- No post-purification needed
- Specify size with +/-10% e.g. 298 +/-12bp
- ‘Bioanalyser’-esque electropherogram
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