Hello,
I'm using Epicentre Ribo-Zero kit for removal of rRNA from Drosophila. I checked RNA on Agilent, before and after depletion, and I observed a picture that seems strange for me (attached). A lot of small RNAs that do not seem to be present in the initial sample and nothing that looks like mRNA peak (it should be about 1000-2000 nt, I guess).
What is your opinion, is it normal?
I'm using Epicentre Ribo-Zero kit for removal of rRNA from Drosophila. I checked RNA on Agilent, before and after depletion, and I observed a picture that seems strange for me (attached). A lot of small RNAs that do not seem to be present in the initial sample and nothing that looks like mRNA peak (it should be about 1000-2000 nt, I guess).
What is your opinion, is it normal?
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