Illumina (MiSeq):
Let' say I have multiple amplicon samples, generated by PCR, that look like:
P7Adapt - pad - Index - pad - GenSpecificPrimerF - ...... - GenSpecificPrimerR - pad - Index2 - pad - P5Adapt
Could I sequence them using the P7Adapt+pad sequence? Instead of having to run mutiple runs and sequence with pad+GenSpecificPrimer each time?
Let' say I have multiple amplicon samples, generated by PCR, that look like:
P7Adapt - pad - Index - pad - GenSpecificPrimerF - ...... - GenSpecificPrimerR - pad - Index2 - pad - P5Adapt
Could I sequence them using the P7Adapt+pad sequence? Instead of having to run mutiple runs and sequence with pad+GenSpecificPrimer each time?
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