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Old 11-11-2009, 12:05 PM   #1
wuhoucdc
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Location: Nashville

Join Date: Oct 2009
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Default The so-call third-generation sequencing coming, but is it credible?

Hi all,

I just read the following paper named Human Genome Sequencing Using Unchained Base Reads on Self-Assembling DNA Nanoarrays from Science Express. In this paper, a sequencing corporation, Complete Genomics, Inc., located at Mountain View, CA announced they will release the so-called third-generation sequecing technology with high coverage (45- to 87-fold coverage per genome) and very low price ($4,400). I was attracted deeply by this paper but with suspicion on this. So I try to contact with this corporation using its service email. But it's unfortunate that I have got any response even though 3 emails were sent until now. So I attach the abstract of this paper here and hope you can perform a evaluation on this so-called new technology. Thank you.

Regards,

Wuhoucdc

PS: Published Online November 5, 2009
Science DOI: 10.1126/science.1181498
Science Express Index

Reports
Submitted on September 3, 2009
Accepted on October 23, 2009



Human Genome Sequencing Using Unchained Base Reads on Self-Assembling DNA Nanoarrays
Radoje Drmanac 1*, Andrew B. Sparks 1, Matthew J. Callow 1, Aaron L. Halpern 1, Norman L. Burns 1, Bahram G. Kermani 1, Paolo Carnevali 1, Igor Nazarenko 1, Geoffrey B. Nilsen 1, George Yeung 1, Fredrik Dahl 2, Andres Fernandez 1, Bryan Staker 1, Krishna P. Pant 1, Jonathan Baccash 1, Adam P. Borcherding 1, Anushka Brownley 1, Ryan Cedeno 1, Linsu Chen 1, Dan Chernikoff 1, Alex Cheung 1, Razvan Chirita 1, Benjamin Curson 1, Jessica C. Ebert 1, Coleen R. Hacker 1, Robert Hartlage 1, Brian Hauser 1, Steve Huang 1, Yuan Jiang 1, Vitali Karpinchyk 1, Mark Koenig 1, Calvin Kong 1, Tom Landers 1, Catherine Le 1, Jia Liu 1, Celeste E. McBride 1, Matt Morenzoni 1, Robert E. Morey 3, Karl Mutch 1, Helena Perazich 1, Kimberly Perry 1, Brock A. Peters 1, Joe Peterson 1, Charit L. Pethiyagoda 1, Kaliprasad Pothuraju 1, Claudia Richter 1, Abraham M. Rosenbaum 4, Shaunak Roy 1, Jay Shafto 1, Uladzislau Sharanhovich 1, Karen W. Shannon 5, Conrad G. Sheppy 1, Michel Sun 1, Joseph V. Thakuria 4, Anne Tran 1, Dylan Vu 1, Alexander Wait Zaranek 4, Xiaodi Wu 6, Snezana Drmanac 1, Arnold R. Oliphant 1, William C. Banyai 1, Bruce Martin 1, Dennis G. Ballinger 1*, George M. Church 4, Clifford A. Reid 1
1 Complete Genomics, Inc., 2071 Stierlin Court, Mountain View, CA 94043, USA.
2 Complete Genomics, Inc., 2071 Stierlin Court, Mountain View, CA 94043, USA.; Present address: Ion Torrent Systems, San Francisco, CA, USA.
3 Complete Genomics, Inc., 2071 Stierlin Court, Mountain View, CA 94043, USA.; Present address: San Diego State University, San Diego, CA, USA.
4 Department of Genetics, Harvard Medical School, Cambridge, MA, USA.
5 Complete Genomics, Inc., 2071 Stierlin Court, Mountain View, CA 94043, USA.; Present address: Life Technologies, Carlsbad, CA, USA.
6 School of Medicine, Washington University, St. Louis, St. Louis, MO, USA.


* To whom correspondence should be addressed.
Radoje Drmanac , E-mail: rdrmanac@completegenomics.com
Dennis G. Ballinger , E-mail: dballinger@completegenomics.com


These authors contributed equally to this work.

Genome sequencing of large numbers of individuals promises to advance the understanding, treatment, and prevention of human diseases, among other applications. We describe a genome sequencing platform that achieves efficient imaging and low reagent consumption with combinatorial probe anchor ligation (cPAL) chemistry to independently assay each base from patterned nanoarrays of self-assembling DNA nanoballs (DNBs). We sequenced three human genomes with this platform, generating an average of 45- to 87-fold coverage per genome and identifying 3.2 to 4.5 million sequence variants per genome. Validation of one genome data set demonstrates a sequence accuracy of about 1 false variant per 100 kilobases. The high-accuracy, affordable cost of $4,400 for sequencing consumables and scalability of this platform enable complete human genome sequencing for the detection of rare variants in large-scale genetic studies.

Last edited by wuhoucdc; 11-11-2009 at 12:52 PM. Reason: Change the title
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Old 11-12-2009, 07:25 AM   #2
dbailey
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Location: CA

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Default

Hi Wuhoucdc,

For some more information about this paper, I encourage you to review our website (http://www.completegenomics.com/data...ngResults.aspx).

If you have some specific questions, we would be happy to address. You can contact us at info@completegenomics.com for general information or at dev-support@completegenomics.com for more detailed analysis support.

Regards,
Dione
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Old 11-12-2009, 08:11 AM   #3
wuhoucdc
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Location: Nashville

Join Date: Oct 2009
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Hi Dione,

After read more carefully on your website about this new technology, I think it will be applied rapidly on various domains. I hope this technology will bring us new chance among human genomics. Thank you.

Best,

Wuhoucdc
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