Hi!
We want to sequence small RNAs and I'm wondering how many reads (or coverage?) are needed for a sample from a mammal to obtain a realistic small RNA profile?
I know that it is possible to multiplex a number of samples in one lane (~60 million reads in total) on the Illumina. Is it reasonable to multiplex, for example, 6 samples in one lane for smallRNAs? This would give me ~10 million reads per sample. However, is this enough or do I need more reads?
Thanks a lot in advance for your help!
Cheers,
Conny
We want to sequence small RNAs and I'm wondering how many reads (or coverage?) are needed for a sample from a mammal to obtain a realistic small RNA profile?
I know that it is possible to multiplex a number of samples in one lane (~60 million reads in total) on the Illumina. Is it reasonable to multiplex, for example, 6 samples in one lane for smallRNAs? This would give me ~10 million reads per sample. However, is this enough or do I need more reads?
Thanks a lot in advance for your help!
Cheers,
Conny
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