I am looking for a FASTA file and GTF file that I can use to align my RNA seq (Hiseq) data and then anotate differential expressed genes. I looked at Xebase database, Ensembel but could not find these files. Can someone point me to a DB where I can find X tropicalis FASTA and GTF/ Gff files.
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Ensembl download page
Species table in the middle of page. Type 'Xenopus' in the Filter box (right side of table header). There are links to download whole genome FASTA, cDNA FASTA, CDS FASTA, etc as well as GTF/GFF3 annotation files.
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
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