1. I download the sra files and get the .qual & .csfasta.
2. get the fastq file using solid2fastq.py.
3. running fastqc on the fastq indicating high content of solid smallRNA adapter.
4. tried both cutadapt and trim galore! on the fastq file:In read named 'SRR1510896.1 1_16_1976_F3': length of quality sequence (50) and length of read (51) do not match
step 4 was run on galaxy!
how can I solve the problem? am I using the wrong trim software?
2. get the fastq file using solid2fastq.py.
3. running fastqc on the fastq indicating high content of solid smallRNA adapter.
4. tried both cutadapt and trim galore! on the fastq file:In read named 'SRR1510896.1 1_16_1976_F3': length of quality sequence (50) and length of read (51) do not match
step 4 was run on galaxy!
how can I solve the problem? am I using the wrong trim software?
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