Im doing whole exome sequencing on Ion torrent platform. I started with 1.4microgram/microliter of stock DNA. The fragmentation of the DNA was done correctly as I can see the smear on E-gel near 130-150basepair. Next I proceed with the adapter ligation of the library and then to the size selection step of the library preparation. On library preparation step I picked up the library of 210 basepair size. Then I proceed with the size selected library amplification. When I checked the Qubit concentration of my amplified library, it was below 4 ng/microliter (required 500ng). This concentration must be near 10 or 11 ng/microlitre. Can anyone tell me where the flaw is in this whole experiment?
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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