SEQanswers

Go Back   SEQanswers > Introductions



Similar Threads
Thread Thread Starter Forum Replies Last Post
free 454 junior kits mehtamorphosis 454 Pyrosequencing 0 04-17-2012 04:48 PM
454 Junior MIDs newendophytologist 454 Pyrosequencing 2 02-24-2012 01:34 PM
ssaha2 with 454 Junior jtrivino Bioinformatics 0 05-13-2011 09:22 AM
new 454 user BobS Introductions 0 08-05-2009 02:15 PM
Hello from a 454 User BeFuPe Introductions 0 03-31-2009 03:29 AM

Reply
 
Thread Tools
Old 07-18-2012, 01:26 AM   #1
Encarnita
Junior Member
 
Location: Tokyo

Join Date: Jul 2012
Posts: 3
Default 454 junior user - hello everyone

Hi everyone,
I am Encarnita, french-spanish immunologist working in Japan.
I am using 454 Lifescience Tech. since more than 3 years, but autonomously using 454 Junior since a year. I've been getting many good infos by surfing on that great forum , but I registered only recently !
I've been suffering several troubles since I perform the 454 libraries preparation, emPCR and sequencing by my self, such as too few enriched beads, too much enriched, never-ending washing steps, few passed filter reads out of many raw reads, software upgrade issues leading to new setup after 12 successfull run....
I have no experience on other platforms, but I am eager to learn and I really want to understand precisely what's going on with the 454 steps.
I'll be pleased to help also, if I have any relevant info.
All the best to seqanswers community !
Encarnita is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 03:02 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2017, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO