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can some one introduce me to next generation sequencing?? chanderbio General 4 08-26-2009 06:36 AM

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Old 09-07-2010, 01:11 PM   #141
evani
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Location: Houston

Join Date: Aug 2010
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Default Hi

Hello All,

I am Uday Evani from Houston. i am bioinformatician who has just entered this field. Currently i am working on ways to improve a home grown indel calling pipeline. i have found seqanswers to be very useful resource and has helped me a lot in getting to know the field. very excited to be here and see you around.

Uday
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Old 09-07-2010, 03:12 PM   #142
sixguns
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Location: Shanghai

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Hello~

I am a new Ph.D. student in UQ, working on deep-sequecing data.
Thanks for SeQanswers, I find our group.
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Old 09-18-2010, 11:36 AM   #143
saikumarkv
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Location: Cincinnati

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Hi everyone,
This forum is awesome. We are just beginning to provide Illumina sequencing services as part of our Genomics Sequencing and Microarray Core at Cincinnati.
sai
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Old 09-21-2010, 10:01 AM   #144
zaclown
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Location: USA

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Hello everybody,

I am a postdoc working on RNAseq; my data come from Illumina genome analyzer II SCS2.6. Glad to have found this forum.
Cheers
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Old 09-22-2010, 02:11 PM   #145
jdanderson
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Location: Davis, CA

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Hello to all,

I am just starting out by getting ready to crunch my first round of RNA-seq data off a GAIIx.
I am very excited and I am becoming increasingly grateful to the creators of this forum. I have already learned a good deal.
The following is a primer on PERL for biologists from one of my professors, Dr. Korf: http://korflab.ucdavis.edu/Unix_and_Perl/index.html
I found it very useful.
See you guys around..
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Old 09-25-2010, 07:53 AM   #146
dmrodz
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Location: Puerto Rico

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Hi all!

I'm a biologist (evolutionary genetics). I work at the Sequencing and Genotyping Facility located at the University of Puerto Rico. We're in the process of buying the Junior 454 from Roche and are waiting for the new Ion Torrent. If anyone has any good or bad comments about the Ion Torrent, they would be most welcome!

Cheers,
D.
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Old 10-08-2010, 10:22 PM   #147
chenjy
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hello, everyone. i am a graduate student and know very little about sequencing. i will often seek help here, and i hope you can be kind enough to help me, thank you.
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Old 10-11-2010, 04:34 AM   #148
wangleibio
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hello ,everyone

Last edited by wangleibio; 10-12-2010 at 02:36 AM.
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Old 10-12-2010, 02:16 PM   #149
jcbreach
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Hello Everyone,
I'm a graduate student working on a transcriptomics-based project and will be using Illumina and/or SOLiD platforms.
Cheers!
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Old 10-14-2010, 10:19 AM   #150
kalidaemon
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Hi everyone
I'm a postdoc in Boston. Working with both ChIP-Seq and RNA-Seq data to understand global differences in estrogen receptor binding and estrogen-mediated gene expression in cardiovascular tissue versus other tissues.
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Old 10-19-2010, 01:11 PM   #151
yangdu
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Location: Waterloo, Canada

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Hello!

I'm a Master's student studying autophagy expression in Drosophila.

I am new to this type of technology, but I am very fascinated with its capabilities and potential, and I hope to use the Illumina sequencing services to assist me in my thesis project

I'm very grateful for this online community!
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Old 10-20-2010, 06:04 AM   #152
chrisaw01
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Hello,

I am a Staff Scientist in a new genomics center for the U.S. Army. We using 454 and Illumina and our focus is on infectious disease genomics. I am pretty new to genomics and hoping to learn a lot from this forum.
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Old 10-20-2010, 09:33 PM   #153
zun
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Location: Japan

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Wink Hi! from Japan

Hi, everyone

I just started analysing RNA-seq on my work.
This site is sooo helpful for beginners like me!

I am excited with discovering new genes or small RNA from NGS data!!

when I will ask some questions on this site,
please help me !!
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Old 10-21-2010, 04:50 AM   #154
chrisaw01
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Quote:
Originally Posted by zun View Post
Hi, everyone

I just started analysing RNA-seq on my work.
This site is sooo helpful for beginners like me!

I am excited with discovering new genes or small RNA from NGS data!!

when I will ask some questions on this site,
please help me !!
Hi Zun,

I also just started a RNA-seq project and would be interested to hear how things progress for you.

Chris
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Old 10-21-2010, 05:23 PM   #155
zun
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Quote:
Originally Posted by chrisaw01 View Post
Hi Zun,

I also just started a RNA-seq project and would be interested to hear how things progress for you.

Chris
Hi, Chris
Thanks for reply

Wow,it seems difficult to define infectious desease genome
because there are so many mutations in it...

Now, I finished bowtie, tophat,cufflinks,cuffcompare to RNA-seq data.
Next, I am going to visualize the result with Gbrowse.

I am also interested in your study !
I am very happy to hear your progress if possible ;b

zun
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Old 10-25-2010, 02:27 PM   #156
Mammon
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Hi all,

I'm a Master's student at the University of Manitoba, Canada. I'm in the department of Medical Microbiology, but I've been thrown into all this bioinformatics stuff! While I find it very interesting, it's also very overwhelming when it comes to the computer side of things. I only have very basic knowledge of HTML, C++, javascript and now I'm slowly learning Perl. I'll be posting lots of questions very soon!
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Old 10-31-2010, 05:16 AM   #157
uday_dd
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Location: Pune, india

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Default Hello All

Hi,
I am Uday Deshpande, working as a Professor Bioinformatics at Bharati University, Pune, India. Currently involved in developing Center of Excellence in Genomics and Proteomics. would like to share experience of next-gen seq data analysis. That why I am here.
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Old 11-01-2010, 10:04 AM   #158
El Mariachi
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Location: China

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Hello, everyone

I am a postgraduate in animal genetics and breeding. The subject I work on is about the copy number variations of domestic animals. The next generation sequecing provides the possibility for the detection of CNVs in the entire genome. Now I am still a newcomer in the field of high-throughput sequencing and I am here to learn.

Best wishes!
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Old 11-02-2010, 08:23 AM   #159
JaKaiser
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Location: Atlanta, GA

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Default Intro

Hi,

Im working with data Sequenced using Illumina's Cluster Station and GA2 with Paired-end Module and SCS v2.3, I've been interested in the process of taking the data from the image files through the workflow into a format for analysis. I have run into alot of issues and questions that Have been handled well by Illumina's Tech Support.

Im hear to learn from other peoples issues and maybe gain more understanding about NexGen Sequencing.

--JaKaiser
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Old 11-14-2010, 04:23 AM   #160
tdoniger
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Location: Israel

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Default Hi

Hi,

I am work in the Bioinformatics Unit at Bar Ilan University,Israel. Most of our users have sequenced their data using illumina. I am fairly new to next generation sequencing and greatly appreciate stumbling across this forum.

Tirza Doniger
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Tirza Doniger, Ph.D.
Bioinformatics Unit
The Mina and Everard Faculty of Life Sciences
Bar Ilan University
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