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Old 03-02-2011, 03:02 AM   #21
RCJK
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If they've just recently expired, they'd still work and you'll be okay.
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Old 03-02-2011, 03:06 AM   #22
LFMar
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Thanks RCJK and Eric.
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Old 10-13-2011, 07:57 AM   #23
vidushi
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hi
i also need a copy of the 454 Titanium Sequencing Manual please? could you kindly send it to me. It will be a great help for me.
email: vidushibeing@gmail.com
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Old 10-13-2011, 11:52 AM   #24
LFMar
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Quote:
Originally Posted by vidushi View Post
hi
i also need a copy of the 454 Titanium Sequencing Manual please? could you kindly send it to me. It will be a great help for me.
email: vidushibeing@gmail.com
I am sending it attached.
Attached Files
File Type: pdf GS-FLX-Titanium-Sequencing-Method-Manual-(May2010).pdf (1.30 MB, 37 views)
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Old 10-13-2011, 08:30 PM   #25
vidushi
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Default NGS data analysis

Thank u so much for the manual.......

can anyone tell me how much time (approx) does it take to analyse the NGS generated data..
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Old 10-13-2011, 08:32 PM   #26
vidushi
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hi

can anyone tell me how much time does it take to analyse data generated by NGS? I am doin PhD, so just curious to know?
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Old 01-12-2012, 06:04 AM   #27
ParkerKB
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Originally Posted by LFMar View Post
Has anyone used adaptors with expired date for 454 rapid library preparation? I've bought the adaptors (up to 10 samples) but I used them for just 2 DNA samples, now I have 8 more samples for library prep and the adaptors date just expired. I don't know if I try to use them or if I buy new ones.
We use a 454 GS Junior and have used expired library prep reagents, and I know of other groups that have used them. We have had problems with library preps both with and without expired reagents so I can't say for sure that it will work, but it is certainly worth a shot. Our technical rep suggested a PCR that amps from the adapters--this will tell you if the adapters have successfully ligated and also to check for the approximate size range of the fragments (since we don't have a bioanalyzer). This would let you know whether it is worth pursuing emPCR rather than going through and not getting any enriched beads (we have found that out the hard way). Let me know if you want the details on the PCR.
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