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Old 05-01-2012, 06:14 AM   #1
chaos81
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Location: miami

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Default pre-small RNA library prep

I am attempting to prep a small rna library and have heard of people selecting for the small rna prior to introduction into protocols. I was wondering if anyone could elaborate on these methods or kits. particularly, I am looking for a method that doesn't involve an acrylamide gel. we have done preps before but tend to get more mRNA and tRNA reads than we would like using the Illumina smRNA kit.

Thanks for any advice that can be given.
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Old 05-03-2012, 06:23 AM   #2
mboth
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I have tried the MirVana Kit from Ambion/LifeTech. That works well and gives you everything below 200nt.
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illumina hiseq, library construction, mirna library, small rna sequencing

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