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Old 12-13-2012, 02:58 AM   #1
carlos
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Location: Spain

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Default Labs that have tried with CLIP-PE

Hi guys,

Do you know any lab that has experience working on Illumina library construction using CLIP-PE methodology (Peng Z, Zhao Z, Nath N, Froula JL, Clum A, et al. (2012) Generation of Long Insert Pairs Using a Cre-LoxP Inverse PCR Approach. PLoS ONE 7(1): e29437. doi:10.1371/journal.pone.0029437)

Regards,

Carlos
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Old 12-13-2012, 04:51 PM   #2
luc
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Yes, we have tried it. Unfortunately without success. We needed something like 24 PCR cycles to get some library from plants. We did not sequence them.
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