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Old 06-17-2013, 05:54 AM   #1
anna_m
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Location: Edinburgh

Join Date: Jun 2013
Posts: 17
Default Size selection for TruSeq DNA libraries

Hello,
When is the best time for carrying out size selection of TruSeq DNA libraries - pre or post PCR? What kind of adapters are labs generally using?

We've been doing a method of using a paired end oligo mix and adding indices during PCR in combination with sizing on the blue pippin. This has worked well for us but when the same is done using the TruSeq adapters and Illumina mastermix/primer cocktail, the final trace is very poor. Screenshot is attached.

Is it the combination of blue pippin and the adapters? Or is it that the size selection should be done after PCR?

Any advice would be much appreciated. Thanks,
Anna.
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